Molecular and Nanoscale Physics

Publications by Lorna Dougan and group

Lenton S, Rhys NH, Towey JJ, Soper AK, Dougan L Highly compressed water structure observed in a perchlorate aqueous solution Nature Communications 8 -, 2017
DOI:10.1038/s41467-017-01039-9
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Wolny M, Batchelor M, Bartlett GJ, Baker EG, Kurzawa M, Knight PJ, Dougan L, Woolfson DN, Paci E, Peckham M Characterization of long and stable de novo single alpha-helix domains provides novel insight into their stability Scientific Reports 7 -, 2017
DOI:10.1038/srep44341
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Alves da Silva M, Lenton S, Hughes M, Brockwell DJ, Dougan L Assessing the potential of folded globular polyproteins as hydrogel building blocks Biomacromolecules 18 636-646, 2017
DOI:10.1021/acs.biomac.6b01877
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Tych KM, Batchelor M, Hoffmann T, Wilson MC, Hughes ML, Paci E, Brockwell DJ, Dougan L Differential effects of hydrophobic core packing residues for thermodynamic and mechanical stability of a hyperthermophilic protein. Langmuir : the ACS journal of surfaces and colloids 32 7392-7402, 2016
DOI:10.1021/acs.langmuir.6b01550
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Lenton S, Walsh DL, Rhys NH, Soper AK, Dougan L Structural evidence for solvent-stabilisation by aspartic acid as a mechanism for halophilic protein stability in high salt concentrations. Physical Chemistry Chemical Physics 18 18054-18062, 2016
DOI:10.1039/c6cp02684b
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Hughes ML, Dougan L The physics of pulling polyproteins: a review of single molecule force spectroscopy using the AFM to study protein unfolding. Reports on Progress in Physics 79 -, 2016
DOI:10.1088/0034-4885/79/7/076601
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Towey JJ, Soper AK, Dougan L Low-Density Water Structure Observed in a Nanosegregated Cryoprotectant Solution at Low Temperatures from 285 to 238 K Journal of Physical Chemistry B 120 4439-4448, 2016
DOI:10.1021/acs.jpcb.6b01185
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Tych KM, Batchelor M, Hoffmann T, Wilson MC, Paci E, Brockwell DJ, Dougan L Tuning protein mechanics through an ionic cluster graft from an extremophilic protein. Soft Matter 12 2688-2699, 2016
DOI:10.1039/C5SM02938D
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Rhys NH, Soper AK, Dougan L Hydrophilic Association in a Dilute Glutamine Solution Persists Independent of Increasing Temperature Journal of Physical Chemistry B 119 15644-15651, 2015
DOI:10.1021/acs.jpcb.5b07413
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Dougan L Surviving at the extremes Physics World 28 30-34, 2015

Hoffmann T, Tych KM, Crosskey T, Schiffrin B, Brockwell DJ, Dougan L Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization ofβ-Barrel Assembly Machinery Polypeptide Transport Associated Domains. ACS Nano 9 8811-8821, 2015
DOI:10.1021/acsnano.5b01962
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Tych KM, Hoffmann T, Batchelor M, Hughes ML, Kendrick KE, Walsh DL, Wilson M, Brockwell DJ, Dougan L Life in extreme environments: Single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms Biochemical Society Transactions 43 179-185, 2015
DOI:10.1042/BST20140274
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Batchelor M, Wolny M, Dougan L, Paci E, Knight PJ, Peckham M Myosin tails and singleα-helical domains Biochemical Society Transactions 43 , 2015
DOI:10.1042/BST20140302
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Dougan L Using Single Molecule Force Spectroscopy to Probe Proteins from Extremophiles BIOPHYSICAL JOURNAL 108 , 2015

Tych KM, Hughes ML, Bourke J, Taniguchi Y, Kawakami M, Brockwell DJ, Dougan L Optimizing the calculation of energy landscape parameters from single-molecule protein unfolding experiments Physical Review E - Statistical, Nonlinear, and Soft Matter Physics 91 -, 2015
DOI:10.1103/PhysRevE.91.012710
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Wolny M, Batchelor M, Knight PJ, Paci E, Dougan L, Peckham M Stable singleα-helices are constant force springs in proteins. J Biol Chem 289 27825-27835, 2014
DOI:10.1074/jbc.M114.585679
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Wolny M, Batchelor M, Parker F, Baboolal T, Mashanov G, Molloy J, Paci E, Dougan L, Knight PJ, Peckham M Unravelling the Properties of Single alpha-Helical Domains in Myosin and other Proteins BIOPHYSICAL JOURNAL 106 , 2014

Towey JJ, Soper AK, Dougan L What happens to the structure of water in cryoprotectant solutions? Faraday Discussions 167 159-176, 2013
DOI:10.1039/c3fd00084b
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Hoffmann T, Tych KM, Hughes ML, Brockwell DJ, Dougan L Towards design principles for determining the mechanical stability of proteins. Phys Chem Chem Phys 15 15767-15780, 2013
DOI:10.1039/c3cp52142g
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Tych K, Dougan L A Single-Molecule Approach to Explore the Role of the Solvent Environment in Protein Folding In Proteins in Solution and at Interfaces: Methods and Applications in Biotechnology and Materials Science , 2013
DOI:10.1002/9781118523063.ch15
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Rhys NH, Dougan L The emerging role of hydrogen bond interactions in polyglutamine structure, stability and association Soft Matter 9 2359-2364, 2013
DOI:10.1039/c2sm27565a
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Hoffmann T, Tych KM, Brockwell DJ, Dougan L Single Molecule Force Spectroscopy Identifies a Small Cold Shock Protein as Being Mechanically Robust The Journal of Physical Chemistry B 117 1819-1826, 2013
DOI:10.1021/jp310442s
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Tych KM, Hoffmann T, Brockwell DJ, Dougan L Single molecule force spectroscopy reveals the temperature-dependent robustness and malleability of a hyperthermophilic protein Soft Matter 9 9016-9025, 2013
DOI:10.1039/C3SM51439K
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Towey JJ, Soper AK, Dougan L Molecular insight into the hydrogen bonding and micro-segregation of a cryoprotectant molecule. J Phys Chem B 116 13898-13904, 2012
DOI:10.1021/jp3093034
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Rhys NH, Soper AK, Dougan L The hydrogen-bonding ability of the amino acid glutamine revealed by neutron diffraction experiments. J Phys Chem B 116 13308-13319, 2012
DOI:10.1021/jp307442f
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Hoffmann T, Tych K, Brockwell DJ, Dougan L A small cold shock protein as a model for force spectroscopy studies of extremophilic proteins FEBS JOURNAL 279 , 2012

Hoffmann T, Dougan L Single molecule force spectroscopy using polyproteins Chemical Society Reviews 41 4781-4796, 2012
DOI:10.1039/c2cs35033e
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Towey JJ, Dougan L Structural examination of the impact of glycerol on water structure Journal of Physical Chemistry B 116 1633-1641, 2012
DOI:10.1021/jp2093862
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Towey JJ, Soper AK, Dougan L Preference for isolated water molecules in a concentrated glycerol-water mixture Journal of Physical Chemistry B 115 7799-7807, 2011
DOI:10.1021/jp203140b
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Dougan L, Crain J, Finney JL, Soper AK Molecular self-assembly in a model amphiphile system. Phys Chem Chem Phys 12 10221-10229, 2010
DOI:10.1039/c003407j
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Fernandez JM, Garcia-Manyes S, Dougan L Force-Clamp Spectroscopy of Single Proteins Single Molecule Spectroscopy in Chemistry, Physics and Biology 96 317-335 572-, 2010
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Dougan L Self-Assembly in a Model Amphiphile System BIOPHYSICAL JOURNAL 98 43A-43A, 2010

Dougan L, Li J, Badilla CL, Berne BJ, Fernandez JM Single homopolypeptide chains collapse into mechanically rigid conformations. Proc Natl Acad Sci U S A 106 12605-12610, 2009
DOI:10.1073/pnas.0900678106
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Garcia-Manyes S, Dougan L, Badilla CL, Brujic J, Fernandez JM Direct observation of an ensemble of stable collapsed states in the mechanical folding of ubiquitin P NATL ACAD SCI USA 106 10534-10539, 2009
DOI:10.1073/pnas.0901213106

Garcia-Manyes S, Dougan L, Fernandez JM Osmolyte-induced separation of the mechanical folding phases of ubiquitin P NATL ACAD SCI USA 106 10540-10545, 2009
DOI:10.1073/pnas.0902090106

Dougan L, Brujic J, Fernandez JM Force-clamp Spectroscopy of Single Proteins, 2009
DOI:10.1002/9783527626137.ch13

Dougan L, Koti ASR, Genchev G, Lu H, Fernandez JM A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions CHEMPHYSCHEM 9 2836-2847, 2008
DOI:10.1002/cphc.200800572

Ainavarapu SRK, Wiita AP, Dougan L, Uggerud E, Fernandez JM Single-molecule force spectroscopy measurements of bond elongation during a bimolecular reaction J AM CHEM SOC 130 6479-6487, 2008
DOI:10.1021/ja800180u

Dougan L, Fernandez JM Tandem repeating modular proteins avoid aggregation in single molecule force spectroscopy experiments J PHYS CHEM A 111 12402-12408, 2007
DOI:10.1021/jp06095+

Walther KA, Grater F, Dougan L, Badilla CL, Berne BJ, Fernandez JM Signatures of hydrophobic collapse in extended proteins captured with force spectroscopy P NATL ACAD SCI USA 104 7916-7921, 2007
DOI:10.1073/pnas.0702179104

Soper AK, Dougan L, Crain J, Finney JL Excess entropy in alcohol-water solutions: A simple clustering explanation J PHYS CHEM B 110 3472-3476, 2006
DOI:10.1021/jp054556q

Dougan L, Hargreaves R, Bates SP, Finney JL, Reat V, Soper AK, Crain J Segregation in aqueous methanol enhanced by cooling and compression J CHEM PHYS 122 -, 2005
DOI:10.1063/1.1888405

Dougan L, Bates SP, Hargreaves R, Fox JP, Crain J, Finney JL, Reat V, Soper AK Methanol-water solutions: A bi-percolating liquid mixture J CHEM PHYS 121 6456-6462, 2004
DOI:10.1063/1.1789951

Dougan L, Crain J, Vass H, Magennis SW Probing the liquid-state structure and dynamics of aqueous solutions by fluorescence spectroscopy JOURNAL OF FLUORESCENCE 14 , 2004

Wolny M, Batchelor M, Bartlett GJ, Baker EG, Kurzawa M, Knight PJ, Dougan L, Takagi Y, Woolfson DN, Paci E, Peckham M Design and Characterization of Long and Stable de novo Single alpha-Helix Domains,